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Experiment bench 1: Changing rules When the system malfunctions, you can tentatively change certain states, one parameter at a time. For example, the problem of limiting the dimensionality of chromatographic peaks can be solved by sequentially changing the mobile phase, changing the protective column, and changing the analytical column. By taking some simple change steps, you may be able to solve the problem.

The rules for the second and second comparison of the experimental platform have already identified the location of the fault or determined a solution to the problem before manual maintenance. In other words, the right solution has already been found before starting. For example, during the injection process, it is found that the peak value of the internal standard has decreased. The injection can be repeated to see how repetitive it is, and if it is an accidental decrease, whether bubbles have been introduced into the quantitative tube. This rule can be used to examine the situation after system changes. After changing the flow, the standard can be injected twice before formal injection to check the stability of retention time and chromatographic peak. If there are excess peaks during gradient elution, empty gradient elution can be performed once (is there really a problem?), using this rule can avoid unnecessary changes and determine corrective measures as soon as possible.

Experiment bench 3: Replacing good components with suspicious ones is a good way to find faults. If you suspect that the detector is causing noise, replace it with a high-performance detector. If the fault is resolved, it indicates that there is a problem with the replaced detector. The scale of application of this rule varies, from replacing the entire component to replacing the integrated block on the printed circuit board.

Experiment bench 4: The replacement rule is used together with the replacement rule. The situation has not improved after the good component replaced the suspicious component, and the original component should be replaced. This approach reduces maintenance costs and prevents the accumulation of used components. This rule only applies to a single fault. The principle of replacement does not apply to the following situations: 1. When removing, the new component has been damaged (such as the pump sealing gasket); 2. Low component price (such as column lined filter); 3. Reinstallation of the original components carries the risk of damage; 4. Components that are regularly replaced.

Experiment bench 5: There are usually two reference conditions for reference condition rules: 1. Standard reference conditions; 2. Test reference conditions. Standard reference conditions, also known as standard test conditions, are conditions that are easy to verify from one system to another, and from one laboratory to another. The data measured using this condition helps to identify issues between actual experiments and systems. If the system pressure increases under certain test conditions and the pressure is normal under standard conditions. This indicates that system anomalies are caused by changes in the laboratory.